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　　人布魯氏菌抗體IgGELISA檢測試劑盒屬固相免疫測定，抗原、抗體的結合只在固相表面上發生。以抗體包被的夾心法為例，加入板孔中的標本，其中的抗原并不是都有均等的和固相抗結合的機會，只有zui貼近孔壁的一層溶液中的抗原直接與抗體接觸。

　　這是一個逐，因此需經擴散才能達到反應的終點。在其后加入的酶標記抗體與固相抗原的結合也同樣如此。這就是為什么ELISA檢測試劑盒反應總是需要一定時間的溫育。

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　　溫育人布魯氏菌抗體IgGELISA檢測試劑盒常采用的溫度有43℃、37℃、室溫和4℃（冰箱溫度）等。37℃是實驗室中常用的保溫溫度，也是大多數抗原抗體結合的合適溫度。在建立ELISA方法作反應動力學研究時，實驗表明，兩次抗原抗體反應一般在37℃經1-2小時，產物的生成可達。

中文名稱：大人布魯氏菌抗體IgGELISA檢測試劑盒
規    格：48孔/人份、96孔/人份，全部為批次
特 異 性：本試劑盒可同時檢測天然或重組的，且與其他相關蛋白無交叉反應
特    點：靈敏性高、特異性強、重復性好
供 應 商：上海哈靈生物科技有限公司

　　為加速反應，可提高人布魯氏菌抗體IgGELISA檢測試劑盒反應的溫度，有些試驗在43℃進行，但不宜采用更高的溫度。抗原抗體反應4℃更為*，在放射免疫測定中多使反應在冰箱中，以形成zui多的沉淀。但因所需時間太長，在ELISA試劑盒中一般不予采用。

　　人布魯氏菌抗體IgGELISA檢測試劑盒保溫的方式除有的ELISA檢測試劑盒儀器附有特制的電熱塊外，一般均采用水浴，可將ELISA試劑盒板置于水浴箱中，ELISA檢測試劑盒板底應貼著水面，使溫度迅速平衡。為避免蒸發，板上應加蓋，也可用塑料貼封紙或保鮮膜覆蓋板孔，此時可讓反應板漂浮在水面上。

　　若用保溫箱，人布魯氏菌抗體IgGELISA檢測試劑盒板應放在濕盒內，濕盒要選用傳熱性良好的材料如金屬等，在盒底墊濕的紗布，zui后將ELISA試劑盒板放在濕紗布上。濕盒應先放在保溫箱中預溫至規定的溫度，特別是在氣溫較低的時候更應如此。無論是水浴還是濕盒溫育，反應板均不宜疊放，以保證各板的溫度都能迅速平衡。

　　室溫溫育的反應，人布魯氏菌抗體IgGELISA檢測試劑盒操作時的室溫應嚴格限制在規定的范圍內，標準室溫溫度是指20-25℃，但具體操作時可根據說明書的要求控制溫育。  室溫溫育時，ELISA檢測試劑盒板只要平置于操作臺上即可。應注意溫育的溫度和時間應按規定力求準確。為保證這一點，一個人操作時，一次不宜多于兩塊板同時測定。

人布魯氏菌抗體IgGELISA檢測試劑盒相關細胞：

293T/17HLX3001DMEM+10%FBS人胚腎細胞
"RT4HLX3002McCOY's 5+10%FBS
人膀胱移行細胞乳頭瘤細胞"
"BHK-21 [C-13]HLX3003MEM培養基10%FBS
倉鼠腎成纖維細胞"
CHLHLX3004DMEM培養基+10%FBS倉鼠肺細胞
"V79HLX3005
 RPMI-1640(GIBCO）+20%FBS
 倉鼠肺細胞"
R 1610HLX3006DMEM+10%FBS倉鼠肺細胞
CHO/dhFr-HLX3007IMDM培養基(GIBCO)+10%FBS倉鼠卵巢細胞,二氫葉酸還原酶缺陷
CHO-K1HLX3008F-12K+10%FBS倉鼠卵巢細胞亞株
"Lec1HLX3009MEMα培養基(GIBCO)+10%FBS
倉鼠卵巢細胞"
CTLA4 Ig-24HLX3010DMEM培養基+10%FBS中國倉鼠卵巢細胞
L6HLX3011DMEM培養基+10%FBS大鼠成肌細胞
"NR8383HLX3012F12K培養基(SIGMA)+20%FBS
大鼠肺泡巨噬細胞"
H9c2(2-1)HLX3013DMEM+10%FBS+1%P/S大鼠心肌細胞
RH-35HLX3014DMEM培養基+10%FBS大鼠肝癌細胞
BRLHLX3015DMEM+10%FBS大鼠肝細胞
BRL 3AHLX3016DMEM培養基+10%FBS大鼠肝細胞
C6HLX3017DMEM+10% FBS大鼠膠質瘤細胞
SHZ-88HLX3018DMEM培養基+10%FBS大鼠乳腺癌細胞
Y3-Ag 1.2.3HLX3019DMEM培養基+10%FBS大鼠骨髓瘤細胞
UMR-106HLX3020DMEM培養基+10%FBS大鼠骨肉瘤細胞
PC-12(高分化)HLX3021RPMI-1640(GIBCO）+10%FBS大鼠腎上腺嗜鉻細胞瘤細胞(高分化)
NRKHLX3022DMEM培養基+10%FBS大鼠腎細胞
NRK-52EHLX3023DMEM培養基+5%FBS大鼠腎細胞
"RBL-2H3HLX3024
 MEM培養基(GIBCO)+15%熱滅活FBS
 大鼠嗜堿性細胞白血病細胞"
RSC96HLX3025DMEM+10%FBS大鼠雪旺細胞
Mv.1.Lu(NBL-7)HLX3026MEM培養基(GIBCO)+10%FBS貂肺上皮細胞
COS-1HLX3027DMEM培養基+10%FBS非洲綠猴SV40轉化的腎細胞
COS-7HLX3028DMEM培養基+10%FBS非洲綠猴SV40轉化的腎細胞
CV-1 [Part of the Wistar Special Collection]HLX3029RPMI-1640(GIBCO）+10%FBS非洲綠猴腎細胞
VeroHLX3030MEM培養基(GIBCO)+10%FBS非洲綠猴腎細胞
VERO C1008 (E6)HLX3031DMEM培養基+10%FBS非洲綠猴腎細胞
FRhK-4HLX3032DMEM培養基+10%FBS恒河猴胚腎細胞
LLC-MK2HLX3033RPMI-1640(GIBCO）+10%FBS恒河猴腎細胞
RF/6AHLX3034RPMI-1640(GIBCO）+10%FBS猴脈絡膜-視網膜(內皮)細胞
B95-8HLX3035RPMI-1640(GIBCO）+10%FBS絨猴EBV轉化的白細胞
OKHLX3036MEM培養基(GIBCO)+10%FBS負鼠腎細胞
MDCK(NBL-2)HLX3037MEM(GIBCO)+10%FBS狗腎細胞
"Super TubeHLX3038D-MEM/F-12培養基(GIBCO)+10%FBS
狗腎細胞"
UMNSAH/DF-1HLX3039DMEM培養基+10%FBS雞胚成纖維細胞
DH82HLX3040MEM培養基(GIBCO)+10%FBS狗腎惡性組織細胞增生癥細胞
CRFKHLX3041RPMI-1640(GIBCO）+10%FBS貓腎細胞
F81HLX3042RPMI-1640(GIBCO）+10%FBS貓腎細胞
EBTr (NBL-4)HLX3043MEM培養基(GIBCO)+10%FBS牛胚氣管細胞
MDBK (NBL-1)HLX3044RPMI-1640(GIBCO）+10%FBS牛腎細胞
Pt K1 (NBL-3)HLX3045MEM培養基(GIBCO)+10%FBS袋鼠腎細胞
PIECHLX3046RPMI-1640(GIBCO）+10%FBS豬髖動脈內皮細胞
NIH/3T3HLX3047DMEM+10% FBS小鼠胚胎細胞
NIH/3T3HLX3047DMEM+10%NBCS(小牛血清）小鼠胚胎細胞
3T6-Swiss albinoHLX3048DMEM培養基+10%FBS小鼠胚胎成纖維細胞
BALB/3T3 clone A31HLX3049DMEM培養基+10%FBS小鼠胚胎成纖維細胞
C3H/10T1/2, Clone 8HLX3050MEM培養基(GIBCO)+10%FBS小鼠胚胎成纖維細胞
Psi2 DAPHLX3051DMEM培養基+10%FBS小鼠胚胎成纖維細胞
C2C12HLX3052DMEM+10%FBS小鼠成肌細胞
A9HLX3053DMEM培養基+10%FBS小鼠皮下結締組織細胞
L Wnt-3AHLX3054DMEM培養基+10%FBS小鼠皮下結締組織細胞
NCTC clone 929 [L cell, L-929, derivative of Strain L]HLX3055MEM(NaHCO3 1.5g/L, Sodium Pyruvate 0.11g/L)+10%FBS小鼠成纖維細胞
B16HLX3056RPMI-1640(GIBCO）+10%FBS小鼠黑色素瘤細胞
B16-F10HLX3057DMEM培養基+10%FBS小鼠皮膚黑色素瘤細胞
MFCHLX3058RPMI-1640(GIBCO）+10%FBS小鼠胃癌細胞
BNL CL.2HLX3059DMEM培養基+10%FBS小鼠胚胎肝細胞
MS1HLX3060DMEM培養基+5%FBS小鼠胰島內皮細胞
Beta-TC-6HLX3061DMEM培養基+15%熱滅活FBS小鼠胰島素瘤胰島β細胞
CT26.WTHLX3062DMEM培養基+10%FBS小鼠結腸癌細胞
K7M2 wt [K7M2-WT]HLX3063DMEM培養基+10%FBS小鼠骨肉瘤成骨細胞
Hepa 1-6HLX3064DMEM+10%FBS小鼠肝癌細胞
"MC3T3-E1 Subclone 14HLX306-MEM培養基+10%FBS
小鼠顱頂前骨細胞亞克隆14"
"OP9HLX3066a-MEM培養基+20%FBS
小鼠骨髓基質細胞"
RAGHLX3067MEM培養基(GIBCO)+10%FBS小鼠腎腺癌細胞
SV40 MES 13HLX3068DMEM培養基+5%FBS小鼠腎小球系膜細胞
Y1HLX3069RPMI-1640(GIBCO）+10%FBS小鼠腎上腺皮質瘤細胞
P815HLX3070DMEM培養基+10%FBS小鼠肥大細胞瘤細胞
RM-1HLX3071RPMI-1640(GIBCO）+10%FBS小鼠前列腺癌細胞
MLTC-1HLX3072RPMI-1640(GIBCO）+10%FBS小鼠睪丸間質細胞瘤細胞
"TM3HLX3073D-MEM/F-12培養基(GIBCO)+5%馬血清+2.5%FBS
小鼠睪丸間質細胞"
F9HLX3074DMEM培養基+10%FBS小鼠畸胎瘤細胞
P19HLX307-MEM（肌醇 43.2mg/l,葉酸 8.82mg/l)+7.5%BCS+2.5%FBS小鼠畸胎瘤細胞
4T1HLX3076RPMI-1640(GIBCO）+10%FBS小鼠乳腺癌細胞
C127HLX3077DMEM培養基+10%FBS小鼠乳腺腫瘤細胞
LLCHLX3078DMEM+10%FBS小鼠Lewis肺癌細胞
S-180HLX3079RPMI-1640(GIBCO）+10%FBS小鼠腹水瘤細胞
Neuro-2a [N2a; Neuro-2a]HLX3080MEM培養基(GIBCO)+10%FBS小鼠腦神經瘤細胞
AtT-20HLX3081RPMI-1640(GIBCO）+10%FBS小鼠垂體瘤細胞
FOHLX3082DMEM培養基+10%FBS小鼠骨髓瘤細胞
P3/NSI/1-Ag4-1 [NS-1]HLX3083DMEM培養基+10%FBS小鼠骨髓瘤細胞
P3X63Ag8HLX3084DMEM培養基+10%FBS小鼠骨髓瘤細胞
P3X63Ag8.653HLX3085RPMI-1640(GIBCO）+10%FBS小鼠骨髓瘤細胞
SP2/0HLX3086RPMI-1640(GIBCO）+10%FBS小鼠骨髓瘤細胞
"P388D1HLX3087RPMI-1640(GIBCO）+90%馬血清+10%FBS
小鼠淋巴樣瘤細胞"
EL4HLX3088RPMI-1640(GIBCO）+10%FBS小鼠淋巴瘤細胞
YAC-1HLX3089RPMI-1640(GIBCO）+10%FBS小鼠淋巴瘤細胞
L5178Y TK+/- clone (3.7.2C)HLX3090DMEM培養基+10%FBS小鼠淋巴瘤細胞
Ana-1HLX3091RPMI-1640(GIBCO）+10%FBS小鼠巨噬細胞
RAW 264.7HLX3092DMEM+10% FBS小鼠單核巨噬細胞白血病細胞
L1210HLX3093DMEM培養基+10%FBS小鼠白血病細胞
L6565HLX3094RPMI-1640(GIBCO）+10%FBS小鼠白血病克隆細胞系
3T3-L1HLX3095DMEM+10%FBS小鼠胚胎成纖維細胞
A-431HLX3096DMEM+10%FBS人表皮癌細胞
HT-1080HLX3097MEM培養基(GIBCO)+10%FBS人纖維肉瘤
"KBHLX3098MEM培養基(GIBCO）+10%FBS
RPMI-1640(GIBCO）+10%FBS
人口腔表皮樣癌細胞"
FaDuHLX3099MEM(GIBCO)+10%FBS人咽鱗癌細胞
"TTHLX3100F-12K+10%FBS
人甲狀腺導管癌細胞"
"SW579 [SW 579; SW-579]HLX3101L-15培養基+10%FBS
人甲狀腺鱗癌細胞"
TE-1HLX3102RPMI-1640(GIBCO）+10%FBS人食管癌細胞
NCI-N87 [N87]HLX3103RPMI-1640(GIBCO）+10%FBS人胃癌細胞
MGC80-3HLX3104RPMI-1640(GIBCO）+10%FBS人胃癌細胞
HGC-27HLX3105RPMI-1640(GIBCO）+20%FBS人胃癌細胞（未分化）
AGSHLX3106F-12+10% FBS人胃腺癌細胞
SGC-7901HLX31071640+10% FBS人胃腺癌細胞
BGC-823HLX31081640+10% FBS人胃腺癌細胞（低分化）
BEL-7402HLX31091640+10% FBS 人肝癌細胞
BEL-7404HLX31101640+10% FBS 人肝癌細胞
Hep 3B2.1-7HLX3111MEM培養基(GIBCO)+10%FBS人肝癌細胞
Hep G2HLX3112DMEM+10% FBS人肝癌細胞
SK-HEP-1HLX3113MEM(GIBCO)+10%FBS人肝癌細胞
SMMC-7721HLX3114DMEM+10% FBS人肝癌細胞
PLC/PRF/5HLX3115MEM培養基(GIBCO)+10%FBS人肝癌亞力山大細胞
Li-7HLX31161640+10%FBS人肝癌細胞
HuH-7HLX3117DMEM+10% FBS人肝癌細胞
HuH-6HLX3118DMEM+10% FBS人肝母細胞瘤細胞
RBEHLX31191640+10% FBS人肝膽管癌細胞
MRC-5HLX3120MEM+10% FBS人胚肺細胞
WI-38HLX3121MEM培養基(GIBCO)+10%FBS人胚肺細胞
THLE-3HLX3122MD 21793的BEGM培養基(目錄號CC3170)人肝永生化細胞
HCCC-9810HLX3123RPMI-1640(GIBCO）+10%FBS人膽管細胞型肝癌細胞
GBC-SDHLX3124RPMI-1640(GIBCO）+10%FBS人膽囊癌細胞
"CFPAC-1HLX3125IMDM(GIBCO）+10%FBS
PANC-1HLX3126DMEM+10% FBS人胰腺癌細胞
SW 1990HLX3127L-15+10%FBS人胰腺癌細胞系
BxPC-3HLX31281640+10% FBS人原位胰腺腺癌細胞
AsPC-1HLX31291640+10% HLXlone滅活血清人轉移胰腺腺癌細胞