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上海科興商貿有限公司
上海科興商貿有限公司
FK31085,5'二硫代雙（2-硝基苯甲酸）98%1g
98%5g
98%25g
98%100g
| FK3108 | 5,5'二硫代雙(2-硝基苯甲酸) | 98% | 1g |
| 98% | 5g | ||
| 98% | 25g | ||
| 98% | 100g |
| Cas號 | 69-78-3 | MDL: | --MFCD00007140 |
| Beilstein | 1896821 | EINECS: | --200-714-4 |
| 分子式 | C14H8N2O8S2； [-SC6H3(NO2)CO2H]2 | ||
| 分子量 | 396.35 | ||
| 別名 | 5,5'二硫代雙(2-硝基苯甲酸) ;雙(3-羧基-4-硝基苯基)二硫，6,6ˊ-二硝基-3,3ˊ-二硫代苯甲酸 ;Ellman’s Reagent (DTNB) | ||
| 分子結構式 |  | ||
| 備注： | |||
| 性狀 | Ellman's Reagent (DTNB) reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution. Ellman's Reagent (5,5'-dithio-bis-[2-nitrobenzoic acid]) is used to estimate sulfhydryl groups in a sample by comparing to a standard curve of a sulfhydryl-containing compound such as cysteine. Alternatively, sulfhydryl groups can be assayed using the extinction coefficient of TNB (14,150M-1cm-1 at 412nm). Ellman's Reagent has been used for applications other than sulfhydryl estimation, such as for determining alkylthiols by HPLC using a pre-column derivatization procedure and to study thiols in the active site of several enzymes. Highlights: •Sulfhydryl assay – reacts quantitatively with free (reduced) sulfhydryl groups (–SH) to yield detectable TNB product •Colorimetric detection – colored product enables spectrophotometric measurement in cuvettes or microplates (λmax = 412nm; ε = 14,150/M•cm) •Proven method – well-characterized chemistry provides quantitation of peptide or protein sulfhydryl groups using extinction coefficient calculation or by comparison to a cysteine standard | ||
| Ellman's Reagent (DTNB) reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution. Ellman's Reagent (5,5'-dithio-bis-[2-nitrobenzoic acid]) is used to estimate sulfhydryl groups in a sample by comparing to a standard curve of a sulfhydryl-containing compound such as cysteine. Alternatively, sulfhydryl groups can be assayed using the extinction coefficient of TNB (14,150M-1cm-1 at 412nm). Ellman's Reagent has been used for applications other than sulfhydryl estimation, such as for determining alkylthiols by HPLC using a pre-column derivatization procedure and to study thiols in the active site of several enzymes. Highlights: •Sulfhydryl assay – reacts quantitatively with free (reduced) sulfhydryl groups (–SH) to yield detectable TNB product •Colorimetric detection – colored product enables spectrophotometric measurement in cuvettes or microplates (λmax = 412nm; ε = 14,150/M•cm) •Proven method – well-characterized chemistry provides quantitation of peptide or protein sulfhydryl groups using extinction coefficient calculation or by comparison to a cysteine standard | |||
| 備注: | 以上描述，僅作參考，并不表示您收到的產品，與此項描述*相符，產品的出廠標準，請參見質量標準。 | ||
| 貯存 | 陰涼干燥處保存 | ||
| 用途 | 巰基測定，是測定蛋白質、多肽和組織中巰基的靈敏試劑。也用于有機磷*藥中毒的監測（*酯酶測定）。 | ||
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