前列腺素F2α(PGF2α)檢測(cè)試劑盒

適用生物 General，通用
前列腺素F2α(PGF2α)檢測(cè)試劑盒檢測(cè)范圍 9.88-800pg/mL 靈敏度 3.95pg/mL
樣本類型 Serum, plasma and other biological fluids
實(shí)驗(yàn)時(shí)長(zhǎng) 2.5h 實(shí)驗(yàn)方法 競(jìng)爭(zhēng)抑制法 前列腺素F2α(PGF2α)檢測(cè)試劑盒規(guī)格 96T
ELISA Kit for Prostaglandin F2 Alpha (PGF2a)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism species General
前列腺素F2α(PGF2α)檢測(cè)試劑盒Sample type Serum, plasma and other biological fluids
Format 96-well strip plate
Assay length 2.5 hours
Detection range 9.88-800pg/mL The standard curve concentrations used for the ELISA’s were 800pg/mL, 266.67pg/mL, 88.89pg/mL, 29.63pg/mL, 9.88pg/mL
Sensitivity The minimum detectable dose of this kit is typically less than 3.95pg/mL.

Specificity
This assay has high sensitivity and excellent specificity for detection of Prostaglandin F2 Alpha (PGF2a).
No significant cross-reactivity or interference between Prostaglandin F2 Alpha (PGF2a) and analogues was observed.
Recovery
Matrices listed below were spiked with 前列腺素F2α(PGF2α)檢測(cè)試劑盒certain level of recombinant Prostaglandin F2 Alpha (PGF2a) and the recovery rates were calculated by comparing the measured value to the expected amount of Prostaglandin F2 Alpha (PGF2a) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 101
EDTA plasma(n=5) 86-102 99
heparin plasma(n=5) 80-101 84

Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostaglandin F2 Alpha (PGF2a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostaglandin F2 Alpha (PGF2a) were tested on前列腺素F2α(PGF2α)檢測(cè)試劑盒 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Prostaglandin F2 Alpha (PGF2a) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-92% 84-98% 86-104% 88-102%
EDTA plasma(n=5) 85-94% 91-103% 94-101% 85-92%
heparin plasma(n=5) 87-98% 98-105% 78-92% 80-92%

Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date 前列腺素F2α(PGF2α)檢測(cè)試劑盒under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120μL Assay Diluent B 1×12mL
Reagent Diluent 1×300μL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50μL standard or sample to each well.
    And then add 50μL prepared Detection Reagent A immediay.
    Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50μL Stop Solution. Read at 450 nm immediay.
Test principle
This assay employs the competitive 前列腺素F2α(PGF2α)檢測(cè)試劑盒inhibition enzyme immunoassay technique. A monoclonal antibody specific to Prostaglandin F2 Alpha (PGF2a) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Prostaglandin F2 Alpha (PGF2a) and unlabeled Prostaglandin F2 Alpha (PGF2a) (Standards or samples) with the pre-coated antibody specific to Prostaglandin F2 Alpha (PGF2a). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the前列腺素F2α(PGF2α)檢測(cè)試劑盒 concentration of Prostaglandin F2 Alpha (PGF2a) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Prostaglandin F2 Alpha (PGF2a) in the sample.