細(xì)胞質(zhì)膜微囊蛋白-1抗體詳細(xì)資料

英文名稱  Anti-Caveolin-1

中文名稱  細(xì)胞質(zhì)膜微囊蛋白-1抗體

別    名  opamine D3 receptor; Caveolin1; Caveolin 1; 3 dopamine receptor; D; Dopamine receptor D3; DRD; ETM1; FET1; Cav-1; ETM1; CAV 1; CAV1.

產(chǎn)品價格  0.1ml/100μg/ 0.2ml/200μg/

濃    度  1mg/1ml

抗體來源  Rabbit

克隆類型  polyclonal

交叉反應(yīng)  hu, rat, mo

產(chǎn)品類型  一抗   

研究領(lǐng)域  信號轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子

蛋白分子量  predicted molecular weight: 20kDa

性    狀  Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human Caveolin-1 C-terminus

亞    型  IgG

純化方法  affinity purified by Protein A

儲 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

產(chǎn)品應(yīng)用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500

（石蠟切片需做抗原修復(fù)）

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

相關(guān)產(chǎn)品  免疫組化常用試劑    免疫印跡常用試劑    各種標(biāo)記二抗 

細(xì)胞質(zhì)膜微囊蛋白-1抗體詳細(xì)資料參考文獻(xiàn)  Pubmed 

產(chǎn)品介紹 The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene. [provided by RefSeq].

Function : May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.

Subunit : Homooligomer. Interacts with GLIPR2, NOSTRIN, SNAP25 and syntaxin. Interacts with rotavirus A NSP4. Interacts (via the N-terminus) with DPP4; the interaction is direct. Interacts with CTNNB1, CDH1 and JUP. Interacts with BMX and BTK.

Subcellular Location : Golgi apparatus membrane;Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Membrane raft. Note=Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.

Tissue Specificity : Expressed in muscle and lung, less so in liver, brain and kidney.

Post-translational modifications : The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.

Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress.

DISEASE : Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.

Similarity : Belongs to the caveolin family.

Database links : UniProtKB/Swiss-Prot: Q03135.4

信號傳導(dǎo)（Signaling Intermediates）

細(xì)胞質(zhì)膜微囊蛋白-1抗體詳細(xì)資料Caveolin-1（小凹蛋白）是細(xì)胞生長相關(guān)信號途徑及腫瘤發(fā)生發(fā)展過程中重要的抑制因子，Caveolae是細(xì)胞膜內(nèi)的特殊膜結(jié)構(gòu)，參與包括細(xì)胞的分子運(yùn)輸、細(xì)胞粘附和信號轉(zhuǎn)導(dǎo)在內(nèi)的多種細(xì)胞活動。Caveolin-1是Caveolae中重要的結(jié)構(gòu)蛋白，抑制細(xì)胞生長，與多種人類腫瘤發(fā)生發(fā)展相關(guān)的信號分子相互作用。Caveolin在信號轉(zhuǎn)導(dǎo)的整合中起支架蛋白的作用。Caveolin構(gòu)成了一個蛋白家族，他們是細(xì)胞質(zhì)膜中發(fā)夾樣結(jié)構(gòu)域的主要結(jié)構(gòu)成分。Caveolin在信號轉(zhuǎn)導(dǎo)的整合中起支架蛋白的作用。至今已經(jīng)鑒定了3種Caveolin （Caveolin-1、2 和3），它們具有不同的組織分布。Caveolin-1涉及上皮細(xì)胞增生的發(fā)病機(jī)制。該抗體可識別α和β型Caveolin-1。

產(chǎn)品圖片  

Overlay histogram showing Mouse kidney cells stained with bs-1453R-PE (red line). The cells were fixed with 1% paraformaldehyde (10 min) and then permeabilized with 1x PBS /0.2%TritonX-100 for 5 min.The cells were then incubated with the antibody (bs-1453R-PE, 2ug/1x106cells) for 30 min at 22-25°C. Isotype control antibody (black line) was rabbit IgG (2ug/1x106cells) used under the same conditions. Acquisition of events were used for analysis