T/G HA-VSMC(人主動脈血管平滑肌細胞)  
 
Cell Biology 
ATCC® Number: CRL-1999™       Price: 
Designations: T/G HA-VSMC  Depositors: RR Gifford 
Biosafety Level: 1 Shipped: frozen 
Medium & Serum: See Propagation Growth Properties: adherent 
Organism: Homo sapiens (human) Morphology: fibroblast
 
 
Source: Organ: aorta
Tissue: smooth muscle
Disease: normal 
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Related Cell Culture Products 
Isolation: (The T/G HA-VSMC line was established from the normal aorta of an 11 month old child.) 
Applications: transfection host (technology from amaxa) 
DNA Profile
(STR): Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 12
D5S818: 12,13
D7S820: 9,10
THO1: 7,8
TPOX: 8
vWA: 16 
Cytogenetic
Analysis: This is a normal human cell line with a 46,XX karyotype. The modal chromosome number was 46, occurring in 90% of cells. The rate of polyploid cells was 0.8%. No structurally altered chromosomes were detected in 15 metaphases karyotyped. Both X chromosomes appeared normal. 
Age: 11 months 
Gender: female 
Ethnicity: Caucasian 
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.05 mg/ml ascorbic acid; 0.01 mg/ml insulin; 0.01 mg/ml transferrin; 10 ng/ml sodium selenite; 0.03 mg/ml Endothelial Cell Growth Supplement (ECGS); fetal bovine serum to a final concentration of 10%, HEPES to a final concentration of 10 mM, TES to a final concentration of 10 mM.
Temperature: 37.0C 
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, and incubate at 37C for 5 minutes. Add an equal volume of fresh medium, disperse the cells and centrifuge to pellet the cells. Resuspend the cells in fresh medium, aspirate and dispense into new flasks.
 
Subc*tion ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
 
Medium renewal: Every 2 to 3 days
 
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature