Human interleukin 18（IL-18）

時間：2011/1/5閱讀：408

Principle of the assay

The kit assay Human IL-18level in the sample，use Purified Human IL-18antibody to coat microtiter plate wells, make solid-phase antibody, then addIL-18to wells,CombinedIL-18 antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Compley,Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed,reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration ofHuman IL-18in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit

1	wash solution	20ml×1bottle	7	Stopp Solution	6ml×1 bottle
2	HRP-Conjugate reagent	6ml×1 bottle	8	Standard（320ng/L）	0.5ml×1 bottle
3	Microelisa stripplate	12well×8strips	9	Standard diluent	1.5ml×1bottle
4	Sample diluent	6ml×1 bottle	10	Instruction	1
5	Chromogen Solution A	6ml×1 bottle	11	Closure plate membrane	2
6	Chromogen Solution B	6ml×1 bottle	12	Sealed bags	1

Specimen requirements

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Human interleukin 18（IL-18）

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