"脂質(zhì)體2000
產(chǎn)品貨號(hào)：YY13629
CAS號(hào)：
英文名稱：Lipofectamine 2000 Transfection Reagent
規(guī)格：BR，
包裝：0.75毫升
產(chǎn)品纖細(xì)資料如下：

脂質(zhì)體2000，上海永葉生物科技有限公司專業(yè)供應(yīng)銷售脂質(zhì)體2000/Lipofectamine 2000 Transfection Reagent,CAS號(hào)！產(chǎn)品訂購(gòu)：,"
 

英文名稱：Lipofectamine 2000 Transfection Reagent；Lipofection 2000
級(jí)別：BR
Lipofectamine 2000 Transfection Reagent is a proprietary cationic lipid formulation that offers the highest transfection efficiencies and protein expression levels on the widest variety of adherent and suspension cell lines. A large percentage of our customers also use this reagent for RNAi gene knockdown experiments. It is the leading transfection reagent on the market for simple, effective, and optimal nucleic acid delivery （Figure 1）. With Lipofectamine 2000 Reagent you will get:
1. Exceptional transfection efficiency in a broad range of cell lines and high levels of recombinant protein expression
2. Superior performance for siRNA delivery （1） （Figure 2）
3. Proven efficacy in the presence of serum-eliminates the need to change media following transfection
4. A reagent proven to work in high-throughput applications
5. A reliable reagent for establishing stable cell lines
Lipofectamine 2000 Reagent works effectively with many primary cell lines and other difficult-to-transfect cell lines （Figure 3, 4, 5）. In addition to its versatility with diverse cell lines, Lipofectamine 2000 allows you to achieve high transfection efficiencies using media with or without serum. For cells sensitive to the absence of serum, add DNA: lipid complexes directly to cells in serum-containing medium. For gene silencing Lipofectamine 2000 Reagent's high-efficiency transfections leads to the high levels of gene knockdown needed to achieve successful results.Simply mix Lipofectamine 2000 Reagent with nucleic acid, add to cell culture, and express protein or knock down genes. The simplicity and speed combined with high transfection efficiency make Lipofectamine™ 2000 Reagent ideal for transient protein expression or high throughput RNAi transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications.Protocols, citations, and additional product information for Lipofectamine 2000 can be found in the Cell Lines Database at www.invitrogen.com⁄celllines.
性狀：液體
用途：生化研究。Lipofectamine™ 2000轉(zhuǎn)染試劑是一種陽(yáng)離子脂質(zhì)體,在體外可以轉(zhuǎn)染許多種哺乳動(dòng)物細(xì)胞，對(duì)各類細(xì)胞系都可提供zui高的轉(zhuǎn)染效率，具有高效性和操作步驟簡(jiǎn)單的優(yōu)點(diǎn)。 其轉(zhuǎn)染效率以及細(xì)胞活性取決于許多實(shí)驗(yàn)因素,如細(xì)胞密度、脂質(zhì)體和DNA的濃度、脂質(zhì)體2DNA復(fù)合物的作用時(shí)間以及是否有其它介質(zhì)成分如抗生素和血清。
使用Lipofectamine™ 2000試劑，您可以獲得：
*在多種細(xì)胞系中具有*地轉(zhuǎn)染效率和較高水平地重組蛋白表達(dá)
*傳輸siRNA的優(yōu)異性能
*在有血清存在的情況下可保證轉(zhuǎn)染效力——轉(zhuǎn)染后無(wú)需更換培養(yǎng)基
*保證可以用于高通量應(yīng)用的試劑
*用于建立穩(wěn)定細(xì)胞系的可靠試劑
Lipofectamine™ 2000試劑為大多數(shù)細(xì)胞提供了zui高的轉(zhuǎn)染效率和活性。當(dāng)存在血清或用于進(jìn)行蛋白表達(dá)的細(xì)胞系（如COS-7，CHO和293）時(shí)，Lipofectamine™ 2000尤為有效，效率可超過(guò)90。是目前應(yīng)用zui廣的轉(zhuǎn)染試劑
保存：2～8℃