產(chǎn)品別名:ACIN 1; ACIN1; ACINU_HUMAN; Acinus; ACN; Apoptotic chromatin condensation inducer 1; Apoptotic chromatin condensation inducer in the nucleus; DKFZp667N107; fSAP152; functional spliceosome-associated protein 152; KIAA0670.
產(chǎn)品規(guī)格:0.1ml、0.2ml、1ml
產(chǎn)品用途:科研實驗
產(chǎn)品價格:請報價
說明書:請?zhí)砑涌头苯铀魅?/p>
抗體來源:該指標有兩種產(chǎn)品,一種是兔來源抗體,一種是鼠來源抗體
克隆類型:兔來源為多克隆抗體,鼠來源單克隆抗體
交叉反應(yīng):請索取說明書查看
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-Acinus抗體,Acinus抗體產(chǎn)品介紹:Chromatin condensation and nuclear fragmentation (CCNF) are the hallmarks of apoptosis. CCNF is triggered by the activation of members of the caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE. A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by Caspase 3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated Acinus L (1341 amino acids), Acinus S (583 amino acids) and Acinus S' (614 amino acids)
原因 解決方法 a 抗體濃度過高或孵育時間過長 降低抗體滴度、抗體孵育時間:室溫1小時或4度過夜 b 孵育溫度過高超過37度 一般在室溫20-28度 c DAB顯色時間過長或濃度過高 顯色時間不超過5-12分鐘,以顯微鏡下觀察為準 2 非特異性背景染色
原因 解決方法 a 操作過程中沖洗不充分 每步?jīng)_洗3次每次5分鐘 b 組織中含過氧化物酶未阻斷 可再配置新鮮3%H2O2封閉孵育時間延長 c 組織中含內(nèi)原性* 正常非免疫動物血清再封閉 d 血清蛋白封閉不充分 延長血清蛋白封閉時間
3 染色弱
原因 解決方法 a 抗體濃度過低、孵育時間過短 提高抗體濃度、孵育時間不能少 于60分鐘 b 試劑超過有效使用時間 應(yīng)更換試劑 c 操作中添加試劑時緩沖液未瀝干 每步滴加試劑前瀝干切片中多余的緩沖液 使試劑稀釋 (應(yīng)防止切片干燥) d 室溫太低 低于15度 要改放在37度孵育箱孵育30-60分鐘或4 度冰箱過夜 e 蛋白封閉過度 封閉不要超過12分鐘
4 染色陰性
原因 解決方法 a 操作步驟錯誤 應(yīng)重試 設(shè)陽性對照 b 組織中無抗原 設(shè)陽性對照以驗證試驗結(jié)果 c 一抗與二抗種屬連接錯誤 仔細確定一抗二抗種屬無誤。