【產品編號】:BYK-11503R 【規 格】:0.1ml/0.2ml 【相關標記】:Alexa Fluor 350、Alexa Fluor 488、Alexa Fluor 555、Alexa Fluor 647、AP、APC、Biotin、Cy3、Cy5、Cy5.5、Cy7、FITC、Gold、HRP、PE、PE-Cy3、PE-CY5、PE-CY5.5、PE-CY7、RBITC. 【濃 度】:1mg/1ml 【抗體來源】:Rabbit or Mouse or Goat 【克隆類型】:polyclonal or monoclonal 【產品類型】:一抗 【產品應用】:可以用于做石蠟切片免疫組化,冰凍切片免疫組化,Elisa,WB,免疫熒光、流式細胞術等實驗。
Anti-SORCS3抗體,液泡蛋白分選受體SORCS3抗體產品介紹:This gene encodes one family member of vacuolar protein sorting 10 (VPS10) domain-containing receptor proteins. The VPS10 domain name comes from the yeast carboxypeptidase Y sorting receptor Vps10 protein. Members of this gene family are large with many exons but the CDS lengths are usually less than 3700 nt. Very large introns typically separate the exons encoding the VPS10 domain; the remaining exons are separated by much smaller-sized introns. These genes are strongly expressed in the central nervous system. Two of the five family members (sortilin and sortilin-related receptor) are synthesized as preproproteins; it is not yet known if this encoded protein is also a preproprotein.
操作流程 1 脫蠟和水化:脫蠟前應將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘。 a 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘 b 無水乙醇中浸泡五分鐘 c 95%乙醇中浸泡五分鐘 d 75%乙醇中浸泡五分鐘 2 抗原修復:用于福爾馬林固定的石蠟包埋組織芯片: A 抗原熱修復 a 高壓熱修復 在沸水中加入EDTA(ph8.0)或0.01m*緩沖溶液(ph6.0).蓋上不銹鋼鍋蓋,但不能鎖定。將玻片置于金屬染色加上,緩慢加壓,是玻片在緩沖液中浸泡五分鐘,然后將蓋子鎖定,小閥門將會升起來。10分鐘后除去熱源,置入涼水中,當小閥門沉下去后打開蓋子。此方法適用于較難檢測或核抗原的抗原修復。 b 沸熱修復 電爐或水浴鍋加熱0.01*緩沖液(ph6.0)至95℃左右,放入組織芯片加熱10-15分鐘。 C 微波爐加熱 在微波爐里加熱0.01*緩沖液(ph6.0)至沸騰后將組織芯片放入,斷電,間隔5-10分鐘,反復1-2次。適用的抗原: Bcl-2.Bax.AR.PR.C-fos.x-jum.z-kit.c-myc.E-cadherin. ChromograninA.Cyclin.ER.Heatshock.Protein.HPV.Ki-67.MDMZ.P53.P34.P15.P-glycoprotein.PKC.PCNA.ras.Rb.等 B 酶消化方法 常用0.1%*和0.4%*液。*使用前預熱37℃,消化時間約為5-30分鐘。適用于被固定遮蔽的抗原:包括 Collagen.GFAP. Complement.Cytokeratin.C-erB-2.LCA.LN.等