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聯(lián)系我時(shí),請(qǐng)告知來(lái)自 環(huán)保在線“Anti-Runx3抗體,Runx3抗體"抗體規(guī)格0.1ml、0.2ml、1ml,抗體產(chǎn)品有效期一年,質(zhì)保三個(gè)月,質(zhì)保期內(nèi)出現(xiàn)任何質(zhì)量問(wèn)題或是出不了實(shí)驗(yàn)結(jié)果均可免費(fèi)退換:
上海研盟生物科技有限公司Anti-Runx3抗體,Runx3抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說(shuō)明書隨貨發(fā)送,您也可以直接我司在線客服索取。客服
【中文名稱】:Runx3抗體
【英文名稱】:Anti-Runx3
【產(chǎn)品編號(hào)】:BYK-0378R
【規(guī) 格】:0.1ml/0.2ml
【相關(guān)標(biāo)記】:Alexa Fluor 350、Alexa Fluor 488、Alexa Fluor 555、Alexa Fluor 647、AP、APC、Biotin、Cy3、Cy5、Cy5.5、Cy7、FITC、Gold、HRP、PE、PE-Cy3、PE-CY5、PE-CY5.5、PE-CY7、RBITC.
【濃 度】:1mg/1ml
【抗體來(lái)源】:Rabbit or Mouse or Goat
【克隆類型】:polyclonal or monoclonal
【產(chǎn)品類型】:一抗
【產(chǎn)品應(yīng)用】:可以用于做石蠟切片免疫組化,冰凍切片免疫組化,Elisa,WB,免疫熒光、流式細(xì)胞術(shù)等實(shí)驗(yàn)。
Anti-Runx3抗體,Runx3抗體產(chǎn)品介紹:RUNX3 binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. May be involved in the control of cellular proliferation and/or differentiation (By similarity). Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain.DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1, Subcellular location in Nucleus.
英文別名:This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
設(shè)置對(duì)照
其目的在于證明和肯定陽(yáng)性結(jié)果的特異性,排除非特異性疑問(wèn)。主要是針對(duì)*抗體對(duì)照,常用的對(duì)照方法包括:1. 陽(yáng)性對(duì)照;2. 陰性對(duì)照;3. 阻斷實(shí)驗(yàn); 4.替代對(duì)照;5. 空白對(duì)照;6. 自身對(duì)照;7. 吸收實(shí)驗(yàn).
1. 陽(yáng)性對(duì)照用已知抗原陽(yáng)性的切片作對(duì)照與待檢標(biāo)本同時(shí)進(jìn)行免疫組織化學(xué)染色,對(duì)照切片應(yīng)呈陽(yáng)性結(jié)果,成為陽(yáng)性對(duì)照。證明全過(guò)程均符合要求,尤其當(dāng)待檢標(biāo)本呈現(xiàn)陰性結(jié)果時(shí),陽(yáng)性對(duì)照尤為重要。
2.陰性對(duì)照用確證不含已知抗原的標(biāo)本作對(duì)照,應(yīng)呈陰性結(jié)果,成為陰性對(duì)照,是陰性對(duì)照的一種。其實(shí)空白、替代、吸收和抑制都屬于陰性對(duì)照。當(dāng)待檢標(biāo)本呈陽(yáng)性結(jié)果時(shí),陰性對(duì)照就更加重要,用以排除假陽(yáng)性。
顯色觀察
免疫酶染色應(yīng)注意控制:
1. 呈色質(zhì)濃度和反應(yīng)時(shí)間。增加成色質(zhì)的量和(或)增加底物反應(yīng)時(shí)間,可增加反應(yīng)產(chǎn)物強(qiáng)度;著色太深可減少反應(yīng)時(shí)間。
2. 過(guò)氧化物酶顯色時(shí),H2O2濃度較大將使顯色反應(yīng)過(guò)快而至背景加深;過(guò)量H2O2可能抑制酶的活性。
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ROCK2 (Rho-associated coiled-coil containing protein kinase 2) Rho相關(guān)蛋白激酶2抗體
RPLP0(Ribosomal protein,large,P0) 酸性核糖體磷蛋白大亞基P0抗體
ROS/c ros(ros protein) 活性氧簇ROS抗體
pRb/p105-Rb(phospho ser780) 磷酸化成視網(wǎng)膜細(xì)胞瘤抑癌蛋白抗體
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