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更新時(shí)間:2017-10-31 15:01:38瀏覽次數(shù):85次
聯(lián)系我時(shí),請(qǐng)告知來自 環(huán)保在線“Anti-P2Y9抗體,G蛋白偶聯(lián)嘌呤受體p2y9抗體"抗體規(guī)格0.1ml、0.2ml、1ml,抗體產(chǎn)品有效期一年,質(zhì)保三個(gè)月,質(zhì)保期內(nèi)出現(xiàn)任何質(zhì)量問題或是出不了實(shí)驗(yàn)結(jié)果均可免費(fèi)退換。:、
中文名稱:G蛋白偶聯(lián)嘌呤受體p2y9抗體
英文名稱:Anti-P2Y9
產(chǎn)品編號(hào):BYK-12074R
別名:5730485F04Rik; G protein coupled receptor 23; G-protein coupled receptor 23; GPR23; LPA receptor 4; LPA-4; LPA4; Lpar4; LPAR4_HUMAN; Lysophosphatidic acid receptor 4; Lysophosphatidic Acid Receptor P2Y9; P2RY9; P2Y purinoceptor 9; P2Y5 like; P2Y5-like receptor; P2Y9; Purinergic receptor 9.
產(chǎn)品介紹:Nucleotides are emerging as important extracellular signaling molecules that mediate several effects, such as proliferation, differentiation , chemotaxis and cytokine release. The P2 receptor family is activated by the binding of nucleotides and is divided into two subfamilies, P2X and P2Y. The P2X receptor family is comprised of ligand-gated ion channels that allow for the increased permeability of calcium into the cell in response to extracellular ATP. The P2Y receptor family are G protein-coupled receptors which mediate the effects of extracellular nucleotides, primarily through the activation of phospholipase C. To some extent, the P2Y receptors can also activate potassium channels or, alternatively, inhibit adenylate cyclase and N-type calcium channels in response to extracellular nucleotides. P2Y9 is activated by lysophosphatidic acid (LPA), a lipid mediator involved in cell proliferation, differentiation, survival and death. In hamsters, P2Y9 mRNA is significantly expressed in ovary tissue compared to other tissues, and innervation with 1-oleoly LPA increases intracellular calcium ion concentration and stimulates adenylyl cyclase activity. P2Y9 is structurally related to nucleotide receptors, and shares 20-24% amino acid homology with the three other LPA receptors (LPA1, LPA2, LPA3).
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
規(guī)格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來源:本公司抗體有兔來源和鼠來源等
克隆類型:兔來源為多抗,鼠來源為單抗
產(chǎn)品類型:一抗
Anti-P2Y9抗體,G蛋白偶聯(lián)嘌呤受體p2y9抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
避免反復(fù)凍融,保質(zhì)期一年。
Anti-P2Y9抗體,G蛋白偶聯(lián)嘌呤受體p2y9抗體合適的抗體稀釋度
抗體的濃度是免疫染色的關(guān)鍵,如果抗體濃度過高,抗體分子過多于抗原決定簇,可導(dǎo)致抗體結(jié)合減少,產(chǎn)生陰性結(jié)果。此陰性結(jié)果并不一定是缺少抗原,而是由于抗體過量,這種現(xiàn)象類似于凝集反應(yīng)中的前帶效應(yīng)(prozone effect)。因此,必須使用一系列稀釋做“棋盤式效價(jià)滴定”,檢測(cè)抗體的合適稀釋度,以得到zui大強(qiáng)度的特異性染色和zui弱的背景染色??贵w稀釋度應(yīng)根據(jù):1.抗體效價(jià)高,溶液中特異性抗體濃度越高,工作稀釋度越高;2.一般講,應(yīng)用的抗體稀釋度越大,溫育時(shí)間越長;3.對(duì)于抗體與非特異性蛋白的結(jié)合,只有高稀釋度時(shí)才能防止其非特異性背景染色;4.稀釋用緩沖液的種類,標(biāo)本的固定和處理過程等也可影響稀釋度,所以合適的稀釋度應(yīng)根據(jù)具體情況測(cè)定??贵w的稀釋主要是指*抗體,因?yàn)?抗體中特異性抗體合適的濃度是關(guān)鍵,應(yīng)用高稀釋度*抗體僅高親和力的特異性染色反應(yīng),減少或消除其中交叉抗體反應(yīng)。
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