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更新時(shí)間:2017-10-31 14:49:52瀏覽次數(shù):191次
聯(lián)系我時(shí),請(qǐng)告知來自 環(huán)保在線“Anti-c-Raf抗體,癌基因c-Raf抗體"抗體規(guī)格0.1ml、0.2ml、1ml,抗體產(chǎn)品有效期一年,質(zhì)保三個(gè)月,質(zhì)保期內(nèi)出現(xiàn)任何質(zhì)量問題或是出不了實(shí)驗(yàn)結(jié)果均可免費(fèi)退換。:、
上海研盟生物科技有限公司Anti-c-Raf抗體,癌基因c-Raf抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取。客服
簡(jiǎn)單介紹:
中文名稱:癌基因c-Raf抗體
英文名稱:Anti-c-Raf
產(chǎn)品編號(hào):BYK-1703R
產(chǎn)品別名:Raf1; c Raf; C-raf; Craf 1 transforming gene; cRaf; cRaf; Craf1 transforming gene; EC 2.7.11.1; Murine sarcoma 3611 oncogene 1; NS5; Oncogene MIL; Oncogene RAF1; OTTHUMP00000160218; OTTHUMP00000207813; OTTHUMP00000209389; Proto-oncogene c-RAF; Raf 1; Raf-1; RAF; Raf proto oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; RAF-1; RAF1_HUMAN; TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV; v raf 1 murine leukemia viral oncogene homolog 1; v-raf murine sarcoma viral oncogene homolog 1; vraf1 murine leukemia viral oncogene homolog 1.
產(chǎn)品規(guī)格:0.1ml、0.2ml、1ml
產(chǎn)品用途:科研實(shí)驗(yàn)
產(chǎn)品價(jià)格:請(qǐng)報(bào)價(jià)
說明書:請(qǐng)?zhí)砑涌头苯铀魅?/p>
抗體來源:該指標(biāo)有兩種產(chǎn)品,一種是兔來源抗體,一種是鼠來源抗體
克隆類型:兔來源為多克隆抗體,鼠來源單克隆抗體
交叉反應(yīng):請(qǐng)索取說明書查看
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲(chǔ) 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-c-Raf抗體,癌基因c-Raf抗體產(chǎn)品介紹:cRaf/Raf1 (RAF proto-oncogene serine/threonine-protein kinase) involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3. The Raf family is comprised of three members (aRaf, bRaf, and cRaf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen activated protein kinase (MAPK) pathway. cRaf kinase is a small GTPase like kinase of 73 kDa, and is a signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Raf1 is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is part of the Ras dependent signaling pathway from receptors to the nucleus.
染色失敗的幾種原因
1.所染的全部切片均為陰性結(jié)果,包括陽性對(duì)照在內(nèi),全部呈陰性反應(yīng),原因可能是:(1)染色未按嚴(yán)格操作步驟進(jìn)行;(2)漏加一種抗體,或抗體失效;(3)緩沖液內(nèi)含*,抑制了酶的活性;(4)底物中所加H2O2量少或失效;復(fù)染或脫水劑使用不當(dāng)。
2.所有切片均呈弱陽性反應(yīng):(1)切片在染色過程中抗體過濃,或干燥了;(2)緩沖液配制中未加氯化鈉或pH不準(zhǔn)確,洗滌不*;(3)使用已變色的呈色底物溶液,或呈色反應(yīng)時(shí)間過長;(4)抗體溫育的時(shí)間過長;(5)H2O2濃度過高,呈色速度過快;(6)粘附劑太厚。
3.所有切片背景過深:(1)未用酶消化處理切片;(2)切片或涂片過厚;(3)漂洗不夠;(4)底物呈色反應(yīng)過久;(5)蛋白質(zhì)封閉不夠或所用血清溶血;(6)使用全血清抗體稀釋不夠。
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Tn-C(Tenascin C) 固生蛋白/細(xì)胞粘合素抗體
TNF-α (Human Tumor Necrosis Factor-α )(mouse monoclonal) 鼠抗腫瘤壞死因子-α單克隆抗體
TNF-α(Tumor Necrosis Factor-α) 腫瘤壞死因子-α抗體
TNFSF4/CD252(Tumor necrosis factor ligand superfamily member 4) 腫瘤壞死因子配體超家族成員4抗體
OX40/CD134/TNFBYK4(Tumor necrosis factor receptor superfamily member 4) CD134抗體
TNF-β(Tumor Necrosis Factor-β) 腫瘤壞死因子-β抗體
TNFAIP1(Tumor necrosis factor-alpha-induced protein 1) 腫瘤壞死因子α誘導(dǎo)蛋白1抗體
TNFSF14/LIGHT/CD258 腫瘤壞死因子配體超家族成員14抗體
TP53(tumor protein p53; Li-Fraumeni syndrome) 抗腫瘤P53抗體
TNFSF18/GITR Ligand(Tumor necrosis factor ligand superfamily member 18) 腫瘤壞死因子配體超家族成員18抗體
HVEM/TNFBYK14(Tumor necrosis factor receptor superfamily member 14) 腫瘤壞死因子受體超家族成員14抗體
*mt(Topoisomerase (DNA) I, mitochondrial) 線粒體DNA拓普西異構(gòu)酶Ⅰ抗體
TP2A/TOP2 alpha(DNA Topoisomerase Ⅱalpha) DNA拓普西異構(gòu)酶ⅡA抗體
TOPOⅡα/DNA TPⅡα (DNA topoisomeraseⅡα) DNA拓普西異構(gòu)酶Ⅱ抗體
TPH (Trptophan YMdroxylase) *羥化酶抗體
TPO (TYMroid Peroxidase) 兔抗甲狀腺過氧化物酶抗體
TRA16(TR4 orphan receptor associated 16 kDa protein) 睪wan激素受體相關(guān)蛋白16抗體
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TRAF2/TNF-R2 (TNF receptor-associated factor 2) 腫瘤壞死因子受體相關(guān)因子2抗體
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