產(chǎn)品介紹:Calponin regulates smooth muscle cell contraction and is a marker of smooth muscle cell differentiation. Calponin, an Actin- and Tropomyosin-binding protein, is characterized as an inhibitory factor of smooth-muscle actomyosin activity. Calponin is implicated in the regulation of smooth muscle contraction through its interaction with F-Actin and inhibition of the Actin-activated MgATPase activity of phosphorylated myosin. Both properties are lost following phosphorylation (primarily at Serine 175) by protein kinase C or calmodulin-dependent protein kinase II. The three forms of Calponin, Calponin 1 (basic Calponin), Calponin 2 (neutral Calponin) and Calponin 3 (acidic Calponin) are found in smooth muscle tissue. Additionally, Calponin 2 is found in heart muscle tissue and Calponin 3 is found in the brain.
Anti-HOXA3抗體,酸性鈣調(diào)蛋白3抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
避免反復凍融,保質(zhì)期一年。
Anti-HOXA3抗體,酸性鈣調(diào)蛋白3抗體微弱的條帶(weak single)
可能存在的原因:蛋白與抗體結(jié)合率低
解決方法:較少洗膜次數(shù)或者縮短洗膜時間;
降低洗膜液中NaCl的濃度(0.15M-0.5M);
降低抗體稀釋液中NaCl的濃度(0.15M-0.5M)
可能存在的原因:抗體量不足
解決方法:抗體和蛋白的結(jié)合較低,可將抗體濃度調(diào)整至初始濃度的2-4倍
可能存在的原因:蛋白量不足
解決方法:增加蛋白上樣量
可能存在的原因:酶和底物時效
解決方法:將酶和底物進行反應,觀察現(xiàn)象,出現(xiàn)異常,則需要重新標記或者購買
可能存在的原因:過期或者陳舊的ECL發(fā)光液
解決方法:更換的新的ECL發(fā)光液
可能存在的原因:脫脂奶粉可能會掩蓋一些抗原
解決方法:降低牛奶的百分比或者用BSA代替
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