Cyanine3 tetrazine,Cy3 tetrazine
Cyanine3 tetrazine is a fluorescent dye with tetrazine moiety for a conjugation reaction called TCO (trans-cyclooctene) ligation. Actually, tetrazines react not only with trans-cyclooctenes, but also with (methyl)cyclopropenes and strained non-benzo-fused cycloalkynes.
Cyanine3 is a moderay hydrophobic dye, enabling labeling in non-aqueous environment as well as in aqueous media containing some organic co-solvent like acetonitrile or DMSO. For reactions in purely aqueous media, a sulfonated version of this reagent is available.
General properties
Appearance: | dark red solid |
Mass spec M+ increment: | 611.4 |
Molecular weight: | 727.64 |
Molecular formula: | C40H46N7BF4O |
Solubility: | good in DCM, DMSO, DMF |
Quality conrol: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
Excitation maximum, nm: | 555 |
ε, L⋅mol−1⋅cm−1: | 150000 |
Emission maximum, nm: | 570 |
Fluorescence quantum yield: | 0.31 |
CF260: | 0.04 |
CF280: | 0.09 |
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伯胺 Primary amine
BDP FL amine
Cyanine3 amine
Cyanine5 amine
Cyanine5.5 amine
Cyanine7 amine
Cyanine7.5 amine
Sulfo-Cyanine5 amine
Sulfo-Cyanine7 amine
