卵母細胞TRO抗體,Anti-Magphinin抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
避免反復凍融,保質期一年。
新學期開學大減價*:
Anti-C5R1 補體成分5受體1抗體
Anti-TrkA/B/C *激酶A/B/C抗體
Anti-TrkA/NTRK1 *激酶A抗體
Anti-TRIM32/BBS11 神經干細胞抑制相關蛋白TRIM32抗體
Anti-omerase Binding Protein, P23 端粒酶結合蛋白P23抗體
Anti-ABLIM3 肌動蛋白結合蛋白LIM3抗體
Anti-TRF2 端粒結合蛋白2抗體
Anti-OLIG3 少突膠質細胞轉錄因子3抗體
Anti-5HT3D+5HT3E Receptor 5-羥色胺受體3D+3E抗體
Anti-Transferrin 轉鐵蛋白/血清鐵傳遞蛋白抗體
Anti-TRBF1/TRF1 端粒體復制結合因子1抗體
Anti-PCDHGA11 原鈣粘蛋白γA11抗體
Anti-Transthyretin/Prealbumin 轉甲狀腺素蛋白抗體
Anti-Desmoglein 2 橋粒芯糖蛋白2抗體
Anti-TRAIL 腫瘤壞死因子相關凋亡誘導配體抗體
Anti-Nfasc186 神經束蛋白186抗體
Anti-TRAF6 腫瘤壞死因子受體相關因子6抗體
Anti-MOBP 少突膠質細胞髓鞘相關蛋白抗體
Anti-TRAF3/CD40bp 腫瘤壞死因子受體相關蛋白3抗體
Anti-Cytokeratin 10+14+17+19+42 細胞角蛋白19,17,14,42,10抗體
卵母細胞TRO抗體,Anti-Magphinin抗體石蠟切片的免疫組化SP法參考步驟:
1.60℃烤片2小時
2.脫蠟。二甲苯脫蠟15分鐘,三次
3.水化。依次經過無水乙醇、95%、90%、80%、70%,各5分鐘,蒸餾水(或自來水)5分鐘
4.PBS洗5分鐘,三次
5.抗原修復。枸櫞酸緩沖液(約92-95℃)煮沸(沸水浴)修復15分鐘,自然涼至室溫。PBS洗5分鐘,三次
6.3%雙氧水消化內源性的過氧化物酶,20分鐘,PBS洗5分鐘,三次
7.正常山羊血清封閉,37℃20分鐘
8.一抗孵育,4℃過ye.
9.PBS洗5分鐘,三次
10.二抗孵育,37℃20分鐘(二抗為*標記的羊抗兔IgG),PBS洗5分鐘,三次
11.三抗孵育,37℃20分鐘(三抗為辣根酶標記的鏈霉親和素),PBS洗5分鐘,三次
12.DAB顯色,鏡下觀察結果,適時終止
13.蘇木素復染5分鐘,自來水沖洗片刻,1%的鹽酸酒精(75%)溶液(請自行配置,配制比例為:100ml 75%酒精+1ml濃鹽酸)分化30秒,自來水沖洗5分鐘藍化
14.脫水。依次經過70%、80%、90%、95%、無水乙醇,各5分鐘,二甲苯15分鐘,三次
15.封片,中性樹膠封片
上海研盟生物科技有限公司Anti-Magphinin抗體*,主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發送,您也可以直接我司在線客服索取。客服:.
中文名稱:卵母細胞TRO抗體
英文名稱:Anti-Magphinin抗體
別 名:KIAA1114; MAGE d3; TROP_HUMAN; MAGE D3 antigen; MAGE superfamily protein; MAGED3; Magphinin; Melanoma Antigen Family D, 3; OTTHUMP00000180629; OTTHUMP00000180636; TRO.
此產品本公司有標記的一抗出售,相關標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
卵母細胞TRO抗體背景介紹:This gene encodes a membrane protein that mediates apical cell adhesion between trophoblastic cells and luminal epithelial cells of the endometrium and is implicated in the initial attachment during the process of embryo implantation. This gene is related to the MAGED gene family by sequence similarity and chromosome location. Multiple alternatively spliced transcript variants have been found for this gene; however, the full-length nature of some variants has not been defined. Trophinin is directly responsible for homophilic cell adhesion, and could be involved with bystin and tastin in a cell adhesion molecule complex that mediates an initial attachment of the blastocyst to uterine epithelial cells at the time of the embryo implantation.
規格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來源:本公司抗體有兔來源和鼠來源等
克隆類型:兔來源為多抗,鼠來源為單抗
產品類型:一抗
抗原修復方法:
方法1:沸水浴修復,將盛有修復液和玻片的燒杯置于沸水浴環境,保持外部沸騰狀態15min,自然冷卻至室溫。
方法2:微波修復,將盛有修復液和玻片的燒杯置于微波爐中,高火5min,停火3min,中火5min,自然冷卻至室溫。
方法3:高壓修復,修復液加入高壓鍋加熱至沸騰,放入玻片,封蓋加壓持續加熱至噴氣時開始計時修復2min,自然冷卻至室溫。
方法4:使用*修復,將*修復液加入到組織上,37℃,消化30min
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