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聯(lián)系我時(shí)，請(qǐng)告知來(lái)自 環(huán)保在線BRaf[21271][100µL]CatalogNumber:21271GeneSymbol:BRAF,BRAF1,RAFB1Description:AntiBRafRabbitPolyclonalAntibodyBackground:BRafisamemberoftheRaffamilyofSer/Thrproteinkinases
BRaf [21271]
[100 µL]
| Catalog Number: 21271 |
| Gene Symbol: BRAF, BRAF1, RAFB1 |
| Description: Anti BRaf Rabbit Polyclonal Antibody |
| Background: BRaf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers. |
| Immunogen: Purified BRaf protein, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:200 |
| Concentration: 0.1mg/ml |
| Host: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Isotype: |
| Purity: Purified from serum |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes BRaf of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Immunohistochemistry: |
 | Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti BRaf polyclonal antibody (Cat. #21271). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti mouse IgG (1:50 dilution) was used as secondary antibody. |
| Related Products | |||
| Catalog# | Name | Size | Price |
| Anti BRaf Rabbit Polyclonal Antibody | 100 μL | ￥2600 | |
| AntiBRaf Mouse Monoclonal Antibody | 100 μL | ￥2800 | |
| Anti BRaf(N581S) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti BRaf(V600E) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti BRaf(V600K) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti BRaf(V600D) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti BRaf(V600R) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti BRaf(G469E) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Publications: |
| 1. Mod Pathol. 2013 Mar;26(3):414-20. |
| 2. Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733 |
| 3. human pathology. February 2015Volume 46, Issue 2, Pages 169–175 |
| 4. human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570 |
| 5. Seminars in Diagnostic Pathology |
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