Cdc42Protein[10107][100µg]CatalogNumber:10107ProductName:Cdc42ProteinSynonyms:Celldivisioncycle42,G25K,CDC42HsSource:Human,recombinantfulllength,His6-tagExpressionHost:E

詳細介紹

Cdc42 Protein [10107]
[100 µg]

Catalog Number: 10107

Product Name: Cdc42 Protein

Synonyms: Cell division cycle 42, G25K, CDC42Hs

Source: Human, recombinant full length, His6-tag

Expression Host: E. coli

Molecular Weight: 21 kDa

Purity: >95% by SDS-PAGE

Introduction: Small GTPases are a super-family of cellular signaling regulators. Cdc42 belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Cdc42, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).

Amino Acid Sequence (1-191)

MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRL
RPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQ
KPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRCVLL

Properties

Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.

Physical Appearance (form): White or clear

Concentration: 1 mg/mL

Storage: -80°C

Preparation Instructions:
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Cdc42 was determined by SDS- PAGE and Coomassie Brilliant Blue Staining.

References:
1. Garrett, W. S. . et al., Cell 102: 325-334, 2000.
2. Irie, F. et al., Nature Neurosci. 5: 1117-1118, 2002.
3. Kawasaki, Y. et al., Oncogene 26: 7620-7627, 2007.
4. Manser, E. et al., Nature 363: 364-367, 1993.
5. Musch, A. et al., EMBO J. 20: 2171-2179, 2001.
6. Nalbant, P. et al., Science 305: 1615-1619, 2004.
7. Shen, Y. et al., Dev. Cell 14: 342-353, 2008.
8. Wu, W. J. et al., Nature 405: 800-804, 2000.
9. Wu, X. et al., Genes Dev. 20: 571-585, 2006.
10. Zheng, Y. et al., J. Biol. Chem. 271: 33169-33172, 1996.

Datasheet:

Publications:

1.
PLoS Pathog. 2013 Jan;9(1):e1003142

2.
Cytokine. 2012 Jan;57(1):158-68

3.
European Journal of Cell Biology Volume 91, Issues 11–12, November–December 2012, Pages 978–987

4.
J Cell Biol. 2012 Aug 20;198(4):677-93

5.
Am J Physiol Lung Cell Mol Physiol. 2011 Jan;300(1):L32-42

6.
Mol Cell Biol. 2011 Nov;31(22):4430-41

7.
PLoS One. 2013 Jul 19;8(7):e70123

8.
PLoS One. 2013 Sep 3;8(9):e73063

9.
J Biol Chem. 2014 Jun 27;289(26):18347-59

10.
United States Patent Application