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Gαi2 Protein Q205L mutant

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Gαi2ProteinQ205Lmutant[10147][10µg]CatalogNumber:10147ProductName:Gαi2ProteinQ205LmutantSynonyms:Guaninenucleotidebindingprotein

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Gαi2 Protein Q205L mutant [10147]
[10 µg]

Catalog Number: 10147

Product Name: Gαi2 Protein Q205L mutant

Synonyms: Guanine nucleotide binding protein, alpha inhibiting activity polypeptide 2, GNAI2, Galphai2

Source: Human, recombinant full length, His6-tag

Expression Host: E. coli

Molecular Weight: 40 kDa

Purity: >95% by SDS-PAGE

Introduction: Heterotrimeric G proteins are critical cellular signal transducers. Gαi represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαi proteins are being explored.

Amino Acid Sequence (1-355, Q205L)

MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECR
QYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEEQGVLPDDLSGVIRRLWAD
HGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGG
LRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDL FEEKITHSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVI IKNNLKDCGLF

Properties

Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl

Physical Appearance (form): White or clear

Concentration: 1 mg/mL

Storage: -80°C

Preparation Instructions:
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Gαi2 Q205L was determined by SDS-PAGE and Coomassie Brilliant Blue Staining.

References:
1. Blatt, C. et al., Proc. Nat. Acad. Sci. 85: 7642-7646, 1988.
2. Bloch, D. B. et al., Am. J. Hum. Genet. 42: 884-888, 1988.
3. Bray, P. et al., Proc. Nat. Acad. Sci. 84: 5115-5119, 1987.
4. Itoh, H. et al., J. Biol. Chem. 263: 6656-6664, 1988.
5. Lan, K.-L. et al., J. Biol. Chem. 273: 12794-12797, 1998.
6. Neer, E. J. et al., Hum. Genet. 77: 259-262, 1987.
7. Ogden, S. K. et al., Nature 456: 967-970, 2008.

Datasheet:

Publications:

1.
Biochem J. 2013 Feb 15;450(1):209-19

2.
J Clin Endocrinol Metab. 2012 Oct;97(10):E1851-61

3.
Cancer Res. 2012 Jan 1;72(1):122-32

4.
Endocrinology. 2012 Oct;153(10):4918-28

5.
Cell Signal. 2011 Mar;23(3):550-4

6.
Biochim Biophys Acta. 2011 Mar;1813(3):466-72

7.
Steroids Volume 77, Issue 5, April 2012, Pages 424–432

8.
J Biol Chem. 2010 Aug 27;285(35):26976-86

9.
Proc Natl Acad Sci U S A. 2010 Jan 12;107(2):628-32

10.
Mol Biol Cell. 2014 Nov 5;25(22):3654-71