CST3Protein[10157][100µg]CatalogNumber:10157ProductName:CST3ProteinSynonyms:CystatinC,Cystatin3,CYSCSource:Human,recombinantfulllength,His6-tagExpressionHost:E

詳細介紹

CST3 Protein [10157]
[100 µg]

Catalog Number: 10157

Product Name: CST3 Protein

Synonyms: Cystatin C, Cystatin 3, CYSC

Source: Human, recombinant full length, His6-tag

Expression Host: E. coli

Molecular Weight: 16 kDa

Purity: >95% by SDS-PAGE

Introduction: Cystatin C, which belongs to the type 2 cystatin gene family, is a potent inhibitor of lysosomal proteinases. It regulates the activity of cathepsin S, a protease with central regulatory functions in retinal pigment epithelial (RPE) cells.

Amino Acid Sequence (1-146)

MAGPLRAPLLLLAILAVALAVSPAAGSSPGKPPRLVGGPMDASVEEEGVRRALDFAVGEYNKASNDM
YHSRALQVVRARKQIVAGVNYFLDVELGRTTCTKTQPNLDNCPFHDQPHLKRKAFCSFQIYAVPWQG
TMTLSKSTCQDA

Properties

Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.

Physical Appearance (form): White or clear

Concentration: 1 mg/mL

Storage: -80°C

Preparation Instructions:
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged CST3 was determined by SDS- PAGE and Coomassie Brilliant Blue Staining.

References:
1. Paraoan, L. et al., Traffic 5: 884-895, 2004.
2. Ratnayaka, A. et al., Exp. Eye Res. 84: 1135-1139, 2007.
3. Zurdel, J. et al., Brit. J. Ophthal. 86: 214-219, 2002.

Datasheet:

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