BRaf(V600K)[26110][100µL]CatalogNumber:26110GeneSymbol:BRAF,BRAF1,RAFB1Description:AntiBRaf(V600K)MouseMonoclonalAntibodyBackground:BRafisamemberoftheRaffamilyofSer/Thrproteinkinases

詳細介紹

BRaf(V600K) [26110]
[100 µL]

Catalog Number: 26110

Gene Symbol: BRAF, BRAF1, RAFB1

Description: Anti BRaf(V600K) Mouse Monoclonal Antibody

Background: BRaf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.

Immunogen: A synthetic peptide from the internal region of Braf(V600K), human origin.

Applications: ELISA, WB, IF, IHC

Recommended Dilutions:
ELISA 1:1000-1:5000
WB 1:500-1:2000
IHC 1:100-1:200

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ ａnd Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes B-Raf V600K, but not wild-type B-Raf proteins from vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of recombinant B-raf proteins. Purified His-tagged BRaf(V600K) protein (amino acids 513-693, lane 2) and corresponding wild type protein (lane 1) were blotted with Anti BRaf(V600K) monoclonal antibody (Cat. # 26110).

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