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武漢紐斯特生物科技有限公司
武漢紐斯特生物科技有限公司
Abl(Y253H)[26154][100µL]CatalogNumber:26154GeneSymbol:ABL1
Abl(Y253H) [26154]
[100 µL]
| Catalog Number: 26154 |
| Gene Symbol: ABL1; ABL; JTK7; bcr-abl; c-ABL; p150; v-abl |
| Description: Anti Abl(Y253H) Mouse Monoclonal Antibody |
| Background: V-abl Abelson murine leukemia viral oncogene homolog 1 also known as ABL1, is a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, stress response and cell migration. Deficient in Abl can cause severe disease such as leukemia. |
| Immunogen: A synthetic peptide from the internal region of Abl which includes the mutation of Y253H, human origin. |
| Applications: ELISA, WB, IHC, IF |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: recognizes Abl Y253H, but not wildtype of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Western blot: |
| Western blot analysis of recombinant Abl(Y253H) and wildtype proteins. Purified His-tagged Abl(Y253H) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-Abl(Y253H) mouse monoclonal antibody (Cat. #26154). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing Abl proteins with anti-Abl(Y253H) antibody. HEK293T cells were transfected with pCDNA3-GFP-Abl (WT) plasmid (left column) or pCDNA3-GFP-Abl(Y253H) plasmid (right column), then fixed and stained with anti-Abl(Y253H) monoclonal antibody (Cat. #26154). |
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