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武漢紐斯特生物科技有限公司
武漢紐斯特生物科技有限公司
BRCA1(C61G)[26319][100ul]CatalogNumber:26319GeneSymbol:BRCA1
BRCA1(C61G) [26319]
[100ul]
| Catalog Number: 26319 |
| Gene Symbol: BRCA1, BRCC1, IRIS, PNCA4, PPP1R53, PSCP |
| Description: Anti BRCA1(C61G) Mouse Monoclonal Antibody |
| Background: BRCA1 is normally expressed in cells of breast and other tissues, where it helps repair damaged DNA or destroy cells if DNA cannot be repaired. It is involved in the repair of chromosomal damage with an important role in the error-free repair of DNA double-strａnd breaks. If BRCA1 itself is damaged by a BRCA1 mutation, damaged DNA is not repaired properly, and this increases the risk for breast cancer. |
| Immunogen: A synthetic peptide from the internal region of BRCA1 which includes the mutation of C61G, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes C61G mutant, but not wild-type BRCA1 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Western blot: |
| Western blot analysis of recombinant BRCA1(C61G) and wildtype proteins. Purified His-tagged BRCA1(C61G) protein (lane2) and corresponding wild-type protein (lane1) were blotted with Anti BRCA1(C61G) monoclonal antibody (Cat. #26319). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing BRCA1 proteins with Anti BRCA1(C61G) antibody. HEK293T cells were transfected with pCDNA3-GFP BRCA1(WT) plasmid (left column) or pCDNA3-GFP BRCA1(C61G) plasmid (right column), then fixed and stained with Anti BRCA1(C61G) monoclonal antibody (Cat. #26319). |
| Related Products | |||
| Catalog# | Name | Size | Price |
| Anti BRCA1(C61G) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti BRCA1(1-120) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti BRCA1(1720-1863) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
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