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武漢紐斯特生物科技有限公司
武漢紐斯特生物科技有限公司
B-RAF(G469E)[26196][100ul]CatalogNumber:26196GeneSymbol:BRAF,BRAF1,RAFB1Description:AntiBRAF(G469E)MouseMonoclonalAntibodyBackground:BRAFbelongstoafamilyofserine-threonineproteinkinases
B-RAF(G469E) [26196]
[100ul]
| Catalog Number: 26196 |
| Gene Symbol: BRAF, BRAF1, RAFB1 |
| Description: Anti BRAF(G469E) Mouse Monoclonal Antibody |
| Background: BRAF belongs to a family of serine-threonine protein kinases. As a part of a signaling pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers. |
| Immunogen: A synthetic peptide from the internal region of BRAF which includes the mutation of G469E, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes G469E mutant, but not wild-type BRAF of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Western blot: |
| Western blot analysis of recombinant BRAF (G469E) and wildtype proteins. Purified His-tagged BRAF (G469E) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti BRAF(G469E) monoclonal antibody (Cat. #26196). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing BRAF proteins with Anti BRAF(G469E) antibody. HEK293T cells were transfected with pCDNA3-GFP BRAF(WT) plasmid (left column) or pCDNA3-GFP-BRAF (G469E) plasmid (right column), then fixed and stained with Anti BRAF(G469E) monoclonal antibody (Cat. #26196). |
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| Publications: |
| 1. Mod Pathol. 2013 Mar;26(3):414-20. |
| 2. Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733 |
| 3. human pathology. February 2015Volume 46, Issue 2, Pages 169–175 |
| 4. human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570 |
| 5. Seminars in Diagnostic Pathology |
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