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武漢紐斯特生物科技有限公司
武漢紐斯特生物科技有限公司
RAS(G12S)[26186][100ul]CatalogNumber:26186GeneSymbol:H-Ras
RAS(G12S) [26186]
[100ul]
| Catalog Number: 26186 |
| Gene Symbol: H-Ras; K-Ras; N-Ras |
| Description: Anti RAS(G12S) Mouse Monoclonal Antibody |
| Background: Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G12S mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions. |
| Immunogen: A synthetic peptide from the internal region of RAS which includes the mutation of G12S, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes G12S mutant, but not wild-type RAS of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Western blot: |
| Western blot analysis of recombinant RAS(G12S) and wildtype proteins. Purified His-tagged RAS(G12S) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti RAS(G12S) monoclonal antibody (Cat. #26186). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing RAS proteins with Anti RAS(G12S) antibody. HEK293T cells were transfected with pCDNA3-GFP Ras(G12S) plasmid, pCDNA3-GFP Ras(WT)plasmid,pCDNA3-GFP Ras(G12S) plasmid, pCDNA3-GFP Ras(G12V)plasmid,pCDNA3-GFP-Ras(G12C)plasmid, pCDNA3-GFP Ras(G12R)plasmid or pCDNA3-GFP Ras(G12A)plasmid, then fixed and stained with Anti RAS(G12S) monoclonal antibody (Cat. #26186). |
| Related Products | |||
| Catalog# | Name | Size | Price |
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| Ras Activation Assay Kit | 20T | ￥6,800 | |
| Anti Ras Rabbit Polyclonal Antibody | 100 μL | ￥2,600 | |
| Anti Ras Mouse Monoclonal Antibody | 100 μL | ￥2,800 | |
| Anti RAS(A146V) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti RAS(K117N) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti RAS(A146P) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti RAS(G13V) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti RAS(G13R) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti Ras(Q61R) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti Ras(Q61L) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti Ras(Q61H) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti HRAS(G13A) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti RAS(G12S) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti RAS(A146T) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti Ras(G13D) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti Ras(G12D) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Publications: |
| 1. Gene Therapy 21, 888-896 (October 2014) |
| 2. J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136 |
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