ActiveRab5GTP[26911][30µL]CatalogNumber:26911GeneSymbol:RAB5Description:AntiActiveRab5MouseMonoclonalAntibodyBackground:Rab5isamemberofRab-familyGTPases

詳細介紹

Active Rab5 GTP [26911]
[30 µL]

Catalog Number: 26911

Gene Symbol: RAB5

Description: Anti Active Rab5 Mouse Monoclonal Antibody

Background: Rab5 is a member of Rab-family GTPases. Rab5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway.

Immunogen: Recombinant full length protein of active Gao

Applications: IP, IHC

Recommended Dilutions: 1 µg for ~2 mg total cellular proteins for IP
1:100 dilution for IHC

Concentration: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG1

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ ａnd Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Rab5 antibody recognizes active Rab5 proteins of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Immunoprecipitation/Western blot:

Rab5 activation assay. Purified full-length Rab5A proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti active Rab5 monoclonal antibody (Cat. No. 26911). Immunoblot was with an anti Rab5 polyclonal antibody (Cat. No. 21151).

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