當(dāng)前位置：武漢紐斯特生物科技有限公司>>試劑盒>>SARS-CoV-2 N protein ELISA Kit

SARS-CoV-2 N protein ELISA Kit

返回列表頁(yè)

參考價(jià)

面議

具體成交價(jià)以合同協(xié)議為準(zhǔn)

產(chǎn)品型號(hào)

品牌

廠商性質(zhì)生產(chǎn)商

所在地

在線詢價(jià) 收藏產(chǎn)品查看聯(lián)系電話

聯(lián)系方式：樂(lè)亮明查看聯(lián)系方式

更新時(shí)間：2024-05-03 08:27:55瀏覽次數(shù)：138次

聯(lián)系我時(shí)，請(qǐng)告知來(lái)自環(huán)保在線

產(chǎn)品分類 品牌分類

  試劑盒

全部產(chǎn)品列表

  其他品牌

武漢紐斯特生物科技有限公司

經(jīng)營(yíng)模式：生產(chǎn)廠家

商鋪產(chǎn)品：415條

所在地區(qū)：湖北武漢市

聯(lián)系人：樂(lè)亮明（經(jīng)理）

詢價(jià) 給他留言

產(chǎn)品簡(jiǎn)介

Product:SARS-CoV-2NproteinELISAKitCatalogNO

詳細(xì)介紹

Product: SARS-CoV-2 N protein ELISA Kit

Catalog NO.: NE-xg-E001

Assay Type: Sandwich

Research Area: Infection immunity

Synonyms: SARS-CoV-2 Nucleoprotein; SARS-CoV-2 NP; SARS-CoV-2 Nucleocapsid protein; SARS-Cov-2 NC; SARS-Cov-2 Protein N; Nucleoprotein; NCP;

Detection Range: 125 -8,000 pg/mL

Sensitivity: 38.74 pg/mL

Standard Preparation

Reconstitute the Standard with 1.0 mL of Universal Diluent, keep for 10 minutes at room temperature, shake gently(not to foam). The concentration of the

standard in the stock solution is 8,000 pg/mL. Please prepare 7 tubes containing 0.5 mL Universal Diluent and produce a double dilution series according to the picture shown below. Mix each tube thoroughly

before the next transfer. Set up 7 points of diluted standard such as 8,000 pg/mL, 4,000 pg/mL, 2,000 pg/mL,

1,000 pg/mL, 500 pg/mL, 250 pg/mL, 125 pg/mL, and the last EP tube with Universal Diluent is the blank as 0 pg/mL.

Typical Data

As the OD values of the standard curve may vary according to the conditions of the objective assay,

the experimenter should fit a standard curve for each test. Typical standard curve provided below is for reference only.

Specificity

This assay has high sensitivity and excellent specificity for detection of SARS-CoV-2 N protein. No significant

cross-reactivity or interference between SARS-CoV-2 N protein ａnd analogues is observed.

Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection

between SARS-CoV-2 N protein and all the analogues, therefore, cross reaction may still exist.

Recovery

Matrices listed below were spiked with certain level of recombinant SARS-CoV-2 N protein and the recovery rates were calculated by comparing the measured value to the expected amount of

SARS-CoV-2 N protein in samples.

Matrix	Recovery range(%)	Average(%)
Serum(n=10)	83-107	95
EDTA plasma(n=10)	82-94	85
Heparin plasma(n=10)	83-99	91

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of

SARS-CoV-2N protein and their serial dilutions. The results were demonstrated by the percentage of calculated

concentration to the expected.

Sample	1:2	1:4	1:8	1:16
Serum(n=10)	83-95%	89-103%	82-99%	79-107%
EDTA plasma(n=10)	92-104%	91-99%	78-98%	80-94%
Heparin plasma(n=10)	86-105%	89-102%	81-97%	85-92%

Precision

Intra-assay Precision (Precision within an assay): Three samples with low, middle and high level SARS-CoV-2 N protein were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): Three samples with low, middle and high level SARS-CoV-2 N protein were tested on 3 different plates, 8 replicates in each plate.

CV (%) = SD/meanX100

Intra-Assay: CV< 10%

Inter-Assay: CV< 12%

Stability

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% within the expiration date under appropriate storage condition.