Description
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Application Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removal endotoxins bound to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies （TSE） research.
Biochem/physiol Actions Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad （Asp39-His69-Ser224）. It is stable in a broad range of environments: pH, buffer salts, detergents （SDS）, and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.
Unit Definition One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C （color by Folin-Ciocalteu reagent）.
Legal Information PicoGreen is a registered trademark of Molecular Probes, Inc.
Properties
grade BioUltra
for molecular biology
form lyophilized powder
mol wt mol wt 28.93 kDa
composition Protein, ≥90% biuret
total impurities ≤500 pg/mg DNA （PicoGreen® assay）
EQP level Elite
foreign activity DNAse, Nickase and RNAse, none detected
storage temp. −20°C
Safety
Personal Protective Equipment dust mask type N95 （US）, Eyeshields, Faceshields, Gloves
Hazard Codes Xn
Risk Statements 36/37/38-42
Safety Statements 22-24-26-36/37
WGK Germany 1
References
reference , Sambrook, J., et al. Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY , （1982）
Sweeney, P.J. and Walker, J.M., Burrell, M.M., Enzymes of molecular biology. Methods Mol. Biol. Towanam NJ , （1993） 16, 306
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鏈霉蛋白酶E 基本信息
中文名稱: 鏈霉蛋白酶E
中文同義詞: 鏈霉蛋白酶E
英文名稱: DISPASE
英文同義詞: STREPTOMYCES GRISEUS NEUTRAL PROTEINASE;STREPTOMYCES GRISENS NEUTRAL PROTEINASE;PROTEASE, CALCIUM ACTIVATED;PROTEASE, NEUTRAL;PRONASE E;NEUTRAL PROTEASE;NEUTRAL PROTEASE, GRADE II;NEUTRAL PROTEASE, GRADE I
CAS號: 78990-62-2
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