Description
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Preparation Note Serum albumin may be referred to as Cohn Fraction V. This naming convention is taken from the original Cohn1 method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Cohn Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.
Properties
assay ≥96% （agarose gel electrophoresis）
form lyophilized powder
mol wt mol wt ~66 kDa
pH 6.5-7.5 （1% in 0.15 M sodium chloride）
storage temp. 2-8°C
Safety
Personal Protective Equipment Eyeshields, Gloves, type N95 （US）, type P1 （EN143） respirator filter
WGK Germany 3
References
Cited Reference 1. Cohn, E.J., et al. J. Am. Chem. Soc. 68, 459, （1946）
Merck Merck 13,8542
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