產品名稱：M-PER Mammalian Protein Extraction Reagent
貨號：Pierce 78501
規格：250ML
Thermo Scientific M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.
The complete cell lysis reagent is a nondenaturing detergent formulation that dissolves cell membranes and extracts total cellular protein in only 5 minutes. M-PER Reagent requires little or no mechanical disruption and yields more protein than freeze/thaw cycles and sonication. This mammalian cell lysis reagent is so efficient that adherent cells do not need to be scraped from the culture dish, enabling easy and direct lysis and analysis of cells grown in 24-well and 96-well plates. Resulting cell lysates from either adherent and suspension cells are compatible with many downstream assays including immunoassays, enzyme assays and a variety of common reporter assays.
Highlights:
Gentle – mild detergent lysis, yielding extracts that are immediay compatible with coomassie （Bradford） and BCA protein assays or SDS-PAGE
Compatible – extracts soluble proteins in nondenatured state, enabling direct use in immunoprecipitation and other affinity purification procedures
Amine-free and dialyzable – formulation ensures compatibility with subsequent assay systems
Convenient – lyse adherent cells directly in plate or after scraping and washing in suspension
Non-denaturing – maintain activity of luciferase, beta-galactosidase, CAT and other reporter genes as well or better than other suppliers' products and freeze/thaw methods
Product Details:
Protein extraction is usually the first key step in any proteomics analysis procedure, and the cells must first be lysed to open the cell and release the proteins of interest. Several methods are commonly used to physically lyse cells, including mechanical disruption, liquid homogenization, sonication, freeze/thaw cycles and manual grinding.