anti-BAX 抗體說明書

【資料簡(jiǎn)介】

 

 anti-BAX 抗體說明書

Catalog Number : Rs-0127R

Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）

Background: The Bcl-2 gene was isolated at the chromosomal breakpoint of t（14;18）-bearing follicular B cell lymphomas（1,2）.Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal （3,5）.Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action （6,7）.A more recently identified protein,designated Bax p21（i.e., Bcl-associated X protein ）,has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2（8）.Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2（8）.

Specificity :

· anti- BAX is a rabbit monoclonal antibody unconjugated

· specific for BAX of human, mo, rat, bovine, sheep, dog, pig

· broadly reactive with mammalian species

· epitope mapping near the amino terminus of BAX of

 human, mo, rat, bovine, sheep, dog, pig origin

· use for western blotting, elisa, immunoprecipitation

 and immunohistochemistry

· Protein G affinity chromatography purification, purity :>95%

· Isotype: IgG

· mol wt: 21kDa

 anti-BAX 抗體說明書

Application:

· Western blotting 1:100-500

· Immunohistochemistry 1:100-500

· ELISA 1:500-1000

· Optimal working dilutions must be determined by the end user.

Storage:

Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

 anti-BAX 抗體說明書